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| Immunogen | A synthesized peptide derived from human Phospho-Phospho-PKR (T451) was used as the immunogen for the Phospho-PKR (Thr451) antibody. |
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Overview
Phospho-PKR (Thr451) Antibody / Protein kinase R / EIF2AK2 is a anti-PKR (phospho-Thr451) Rabbit antibody Recombinant Rabbit Monoclonal clone 32E50 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: PKR (phospho site: Thr451)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32E50, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Phospho-PKR (Thr451) antibody is widely applied in virology, immunology, and stress signaling research. By detecting PKR activation, researchers can evaluate how cells respond to viral infection and other stressors such as interferon signaling, oxidative stress, and endoplasmic reticulum stress. Phosphorylation at Thr451 provides a reliable marker of PKR kinase activity and initiation of antiviral defense pathways.
Western blot assays reveal phosphorylated PKR bands distinct from unphosphorylated protein. Immunohistochemistry highlights PKR activation in infected or stressed tissues, while immunofluorescence demonstrates subcellular localization of phospho PKR in cytoplasmic foci and stress granules. These methods provide detailed insight into antiviral signaling and translational control.
PKR phosphorylation at Thr451 integrates innate immunity and apoptosis. Persistent activation induces cell death, contributing to tissue pathology during infection and chronic disease. Dysregulation of PKR activity has been implicated in cancer, metabolic disorders, and neurodegenerative disease. By applying Phospho-PKR (Thr451) antibody, scientists can investigate the balance between protective antiviral signaling and detrimental chronic activation.
PKR also modulates inflammatory pathways including NF-kB activation and cytokine production. Through its ability to couple stress responses to immune activation, PKR influences outcomes in infection, autoimmunity, and tumor immunity. The phospho-specific antibody therefore provides a versatile tool for studying immune regulation and therapeutic targeting.
Phospho-PKR (Thr451) antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
