| Field | Specification |
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| Immunogen | A synthesized peptide derived from human Phospho-Protein Kinase D2 (S876) was used as the immunogen for the Phospho-Protein Kinase D2 (Ser876) antibody. |
| Isotype | |
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| Reactivity | |
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Overview
Phospho-Protein Kinase D2 (Ser876) Antibody / PRKD2 is a anti-D2 (phospho-Ser876) Rabbit antibody Recombinant Rabbit Monoclonal clone 32P58 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: D2 (phospho site: Ser876)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P58, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Protein kinase D2 plays a central role in diverse cellular pathways, including vesicle trafficking, oxidative stress responses, and cytoskeletal remodeling. Studies with Phospho-Protein kinase D2 (Ser876) antibody have shown that phosphorylation at serine 876 occurs within the activation loop of the kinase domain, stabilizing active conformations and promoting downstream signaling. Activation of PRKD2 influences nuclear factor kappa B activity, gene expression, and secretion of cytokines. This modification thus represents a key regulatory switch linking extracellular signals to transcriptional responses.
PRKD2 has been implicated in oncogenesis, cardiovascular disease, and immune regulation. Research using Phospho-Protein kinase D2 (Ser876) antibody has revealed that hyperactivation of PRKD2 promotes tumor growth, angiogenesis, and metastasis by stimulating survival and motility pathways. In the cardiovascular system, phosphorylation of PRKD2 influences contraction, vascular permeability, and stress responses. Its role in immune signaling involves regulation of T cell receptor pathways and cytokine secretion. The breadth of these functions underscores the importance of monitoring phosphorylation status.
Beyond physiological roles, PRKD2 has become a potential therapeutic target. Small molecule inhibitors that block PRKD activation have been investigated in preclinical cancer and inflammatory models. Phospho-Protein kinase D2 (Ser876) antibody is a valuable tool in assessing drug effects by specifically detecting activation state. Such reagents allow researchers to dissect how therapies modulate phosphorylation dependent PRKD2 signaling and how this impacts cellular outcomes.
Phospho-Protein kinase D2 (Ser876) antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated from total PRKD2, while immunohistochemistry reveals activation patterns in tissue samples. Immunofluorescence demonstrates dynamic localization changes upon stimulation, providing insight into subcellular distribution of active kinase. These applications make the antibody essential for signaling research.
By supplying validated Phospho-Protein kinase D2 (Ser876) antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
