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| Immunogen | A synthesized peptide derived from human Phospho-Tuberin (S939) was used as the immunogen for the Phospho-Tuberin (Ser939) antibody. |
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Overview
Phospho-Tuberin (Ser939) Antibody / TSC2 is a anti-TSC2 (phospho-Ser939) Rabbit antibody Recombinant Rabbit Monoclonal clone 32T70 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: TSC2 (phospho site: Ser939)
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32T70, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC
Biological background
Tuberin integrates upstream signals from growth factors, nutrients, and energy status. Akt phosphorylates Tuberin at multiple residues including serine 939, leading to inhibition of the TSC1 TSC2 complex and activation of mTORC1. Research using Phospho-Tuberin (Ser939) antibody has shown that phosphorylation reduces the ability of Tuberin to suppress Rheb, resulting in increased protein synthesis and cell growth. This post translational modification thus acts as a switch between anabolic and catabolic states, linking extracellular signals to metabolic control.
Mutations in TSC2 cause Tuberous sclerosis complex, a genetic disorder characterized by benign tumor formation in multiple organs, epilepsy, and cognitive impairment. While disease is typically associated with truncating mutations, dysregulation of phosphorylation also impacts Tuberin activity. Studies with Phospho-Tuberin (Ser939) antibody have demonstrated that aberrant Akt signaling leads to hyperphosphorylation, promoting uncontrolled mTORC1 activation and contributing to tumorigenesis. This has made the TSC1 TSC2 pathway a target for therapies such as mTOR inhibitors, which are now widely used in clinical management of Tuberous sclerosis complex and related conditions.
Beyond inherited disease, phosphorylation of Tuberin has implications in sporadic cancers, metabolic syndromes, and neurodevelopmental disorders. Overactive Akt driven phosphorylation at serine 939 contributes to tumor growth and survival in breast cancer, prostate cancer, and glioblastoma. Research using Phospho-Tuberin (Ser939) antibody allows detection of these changes, providing a biomarker for pathway activation. In metabolic disease, altered mTOR regulation contributes to insulin resistance and type 2 diabetes, further expanding the relevance of Tuberin phosphorylation in human health.
Phospho-Tuberin (Ser939) antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated from non phosphorylated protein, providing a measure of pathway activity. Immunohistochemistry highlights tissue specific distribution of phosphorylated Tuberin in disease samples, while immunofluorescence visualizes subcellular localization changes after growth factor stimulation. Together, these approaches help define how Tuberin phosphorylation controls mTORC1 signaling in diverse cellular contexts.
By supplying Phospho-Tuberin (Ser939) antibody,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Quantifying post-translational regulation (including phosphorylation) alongside total protein levels.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Phospho-site readouts are condition-dependent and are often compared to total target levels when available.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
