| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E. coli-derived recombinant human protein (amino acids I188-Q430) was used as the immunogen for the PHTF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PHTF1 Antibody / Putative homeodomain transcription factor 1 is an antibody targeting PHTF1, raised in Rabbit for protein detection and localization studies where these specifications are required.
Key elements and design rationale
- Target: PHTF1.
- Antibody identity: Polyclonal (rabbit origin); Rabbit IgG.
- Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
- Format: Antigen affinity purified.
- Species reactivity: Human.
- Listed applications: WB, FACS, Direct ELISA (refer to on-page specifications for application-specific guidance).
Biological background
PHTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alternate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.
Research relevance and current trends
- Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
- Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
- Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.
Common research applications
- Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- Flow cytometry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
- ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.
Notes for experimental interpretation
- Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
- Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
- Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
- Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
