| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PHYHD1 recombinant protein (Position: M1-Q250) was used as the immunogen for the PHYHD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PHYHD1 Antibody / Phytanoyl-CoA dioxygenase domain-containing protein 1 is a anti-PHYHD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PHYHD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Functionally, PHYHD1 antibody identifies a 290-amino-acid protein localized mainly in the cytoplasm and potentially associated with peroxisomes. PHYHD1 contains a conserved 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase domain that catalyzes oxidation reactions using molecular oxygen and 2-oxoglutarate as cofactors. Although its precise substrates remain under investigation, PHYHD1 may regulate lipid processing, hypoxia response, and reactive oxygen species signaling.
The PHYHD1 gene is located on chromosome 9q34.11 and is expressed in brain, liver, and kidney. Transcription of PHYHD1 is induced by cellular stress and metabolic cues, suggesting it plays a role in adaptive metabolic regulation. Comparative genomics studies indicate conservation across vertebrates, implying fundamental metabolic importance.
Pathologically, alterations in PHYHD1 expression have been associated with gliomas and metabolic disorders involving peroxisomal dysfunction. Reduced PHYHD1 levels may impair fatty acid oxidation and redox homeostasis, while overexpression correlates with enhanced metabolic flexibility in cancer cells. Research using PHYHD1 antibody supports studies in lipid oxidation, metabolic adaptation, and enzymatic regulation under hypoxia.
PHYHD1 antibody is validated for western blotting, immunofluorescence, and ELISA to detect 2OG-dependent oxygenases.
Structurally, Phytanoyl-CoA dioxygenase domain-containing protein 1 features a double-stranded beta-helix fold typical of 2OG oxygenases, with a conserved His-Asp-His metal-binding triad that coordinates Fe(II). This structure enables catalytic hydroxylation reactions essential for lipid and oxygen metabolism. This antibody facilitates analysis of PHYHD1's role in oxidative biochemistry, peroxisomal function, and metabolic stress response.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
