| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PIK3CA; Phosphatidylinositol 4; 5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bispho |
| Clonality | |
| Concentration | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PI 3-kinase p110alpha. AA range:470-519 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
Primary AntibodyThis antibody targets PIK3CA and detects endogenous levels of PIK3CA protein.
Validated Applications
| Application | Dilution Guidance |
|---|---|
| Immunofluorescence (IF) | Refer to datasheet for recommended working dilution |
| Western Blot (WB) | Refer to datasheet for recommended working dilution |
| Immunohistochemistry (IHC) | Refer to datasheet for recommended working dilution |
| ELISA | Refer to datasheet for recommended working dilution |
Antibody Specifications
| Target | PIK3CA |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Purification | Affinity Purification |
| Molecular Weight | ~124.3 kDa |
| Concentration | 1 mg/ml |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PI 3-kinase p110alpha. AA range:470-519 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 5290; Mouse: 18706 |
| UniProt | P42336 (Human: P42336; Mouse: P42337) |
Scientific Background
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha encoded by PIK3CA represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns (4,5) P2. PIK3CA has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of PIK3CA has been defined on chromosome 22
Alternative Names
PIK3CA; Phosphatidylinositol 4; 5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bispho
Safety & Handling
| Target | PIK3CA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Reactivity | Human, Mouse |
| Purification | Affinity Purification |
| Molecular Weight | ~124.3 kDa |
| Concentration | 1 mg/ml |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PI 3-kinase p110alpha. AA range:470-519 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage | -20°C for 1 year |
| Gene ID | Human: 5290; Mouse: 18706 |
| UniProt | P42336 (Human: P42336; Mouse: P42337) |
Optimized working dilutions depend on sample type and detection system. Titration is recommended.
| Application | Dilution Note |
|---|---|
| Immunofluorescence (IF) | Refer to datasheet for recommended working dilution |
| Western Blot (WB) | Refer to datasheet for recommended working dilution |
| Immunohistochemistry (IHC) | Refer to datasheet for recommended working dilution |
| ELISA | Refer to datasheet for recommended working dilution |
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha encoded by PIK3CA represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns (4,5) P2. PIK3CA has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of PIK3CA has been defined on chromosome 22
Alternative Names
PIK3CA; Phosphatidylinositol 4; 5-bisphosphate 3-kinase catalytic subunit alpha isoform; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; Phosphatidylinositol 4,5-bispho
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
