| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PICALM was used as the immunogen for the PICALM antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PICALM Antibody / Phosphatidylinositol binding clathrin assembly protein is a anti-PICALM Rabbit antibody Recombinant Rabbit Monoclonal clone 32P30 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: PICALM
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 32P30, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
PICALM antibody is widely applied in neuroscience, immunology, and oncology. In neurons, PICALM participates in synaptic vesicle endocytosis, supporting neurotransmission. In hematopoietic cells, it regulates receptor mediated endocytosis and antigen presentation. Genetic variants in PICALM are strongly associated with Alzheimer disease risk, making it a focus of neurodegenerative research. By detecting PICALM, researchers can investigate how clathrin mediated endocytosis impacts diverse physiological and pathological processes.
Western blotting with PICALM antibody detects protein expression in brain and immune cell lysates. Immunohistochemistry maps distribution in neuronal tissues and lymphoid organs. Immunofluorescence highlights punctate localization at clathrin coated pits and endosomes. These assays allow detailed analysis of PICALM function in vesicular trafficking.
PICALM is also important in oncology. Chromosomal translocations involving PICALM, such as PICALM AF10 fusions, are associated with acute leukemias. These fusions alter transcriptional programs and contribute to leukemogenesis. Detection of PICALM with antibody based methods supports research into oncogenic mechanisms and potential diagnostic strategies. In solid tumors, altered PICALM expression affects endocytosis of growth factor receptors and signaling pathways, influencing tumor progression.
In Alzheimer disease, genome wide association studies have identified PICALM variants as risk factors. Mechanistically, PICALM regulates endocytosis of amyloid precursor protein and clearance of amyloid beta. Altered PICALM expression affects synaptic function and neuronal survival, linking endocytosis defects to neurodegeneration. By applying PICALM antibody, scientists can explore these mechanisms and evaluate therapeutic strategies targeting endocytic pathways.
PICALM also contributes to immune regulation. In antigen presenting cells, it modulates receptor trafficking and signaling. Its role in membrane dynamics extends to viral infections, where viruses exploit clathrin mediated endocytosis for entry. PICALM antibody therefore provides a tool for studying both host defense and viral pathogenesis.
Beyond its cellular functions, PICALM has structural features that enable its roles in trafficking. It contains phosphatidylinositol binding domains and clathrin binding motifs, which link membrane lipids to clathrin coat assembly. It also interacts with adaptor proteins and other regulators of endocytosis. These interactions highlight the versatility of PICALM as a scaffold protein coordinating vesicle formation.
PICALM antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
