| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PICK1 recombinant protein (Position: A41-E376) was used as the immunogen for the PICK1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PICK1 Antibody / Protein interacting with C kinase 1 is a anti-PICK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PICK1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, PICK1 antibody identifies a 415-amino-acid cytoplasmic protein containing a PDZ domain and a BAR domain. The PDZ domain mediates interactions with the C-termini of target proteins such as AMPA-type glutamate receptors, while the BAR domain facilitates membrane curvature sensing and vesicle formation. PICK1 coordinates PKC-dependent receptor internalization and recycling at synapses.
The PICK1 gene is located on chromosome 22q13.1 and is widely expressed in brain, testis, and endocrine tissues. In neurons, PICK1 regulates trafficking of AMPA and metabotropic glutamate receptors, influencing long-term synaptic plasticity. It also interacts with ion channels, transporters, and polarity proteins to maintain signal compartmentalization.
Pathologically, dysregulation of PICK1 contributes to neurological and psychiatric disorders including epilepsy, schizophrenia, and drug addiction. Altered PICK1 expression affects synaptic strength and receptor turnover, disrupting excitatory signaling balance. Research using PICK1 antibody supports studies in synaptic transmission, membrane trafficking, and PKC signaling.
PICK1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect BAR domain scaffold proteins and synaptic trafficking regulators.
Structurally, Protein interacting with C kinase 1 features a PDZ domain that binds short peptide motifs on target proteins and a BAR domain that dimerizes to shape membranes. This antibody enables analysis of PICK1's regulatory function in receptor dynamics and neural signaling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
