| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PIP4K2B recombinant protein (Position: D116-K360) was used as the immunogen for the PIP4K2B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PIP4K2B Antibody / Phosphatidylinositol-5-phosphate 4-kinase type-2 beta is a anti-PIP4K2B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PIP4K2B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PIP4K2B is encoded by the PIP4K2B gene located on human chromosome 17q11.2. The protein is approximately 416 amino acids in length and belongs to the type II phosphatidylinositol 4-kinase family. PIP4K2B localizes to both the cytoplasm and nucleus, dynamically shuttling in response to growth factor stimulation and metabolic status. Its nuclear localization is critical for maintaining gene expression through phosphoinositide-dependent transcriptional regulation.
The PIP4K2B antibody detects a 47 kilodalton protein by western blot and reveals cytoplasmic and nuclear staining under confocal microscopy. PIP4K2B cooperates with its isoforms PIP4K2A and PIP4K2C to maintain phosphoinositide homeostasis. It modulates nuclear PIP2 levels, influencing chromatin remodeling, mRNA processing, and DNA damage responses. In the cytoplasm, PIP4K2B regulates endosomal trafficking and actin cytoskeleton reorganization, integrating lipid metabolism with cell motility.
Functionally, PIP4K2B connects metabolic signaling with growth control through crosstalk between phosphoinositide and mTOR pathways. Loss of PIP4K2B sensitizes cells to metabolic stress and DNA damage, while its overexpression enhances proliferation and survival in cancer cells. Mutations or reduced expression of PIP4K2B have been linked to metabolic disorders, insulin resistance, and neurodegeneration.
Because of its dual role in lipid signaling and nuclear regulation, PIP4K2B is a key node in phosphoinositide-mediated signal integration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
