| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | E.coli-derived human PIP5K1A recombinant protein (Position: I33-H562) was used as the immunogen for the PIP5K1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PIP5K1A Antibody / Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha is a anti-PIP5K1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic, cell membrane.
Key elements and design rationale
- Target: PIP5K1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
Functionally, PIP5K1A antibody identifies a 562-amino-acid enzyme that catalyzes the phosphorylation of phosphatidylinositol 4-phosphate at the D5 position, generating PI(4,5)P2 at the plasma membrane. This phospholipid acts as a precursor for multiple signaling molecules, including IP3, DAG, and PI(3,4,5)P3, and serves as a membrane docking site for proteins involved in vesicle trafficking, actin remodeling, and receptor signaling. PIP5K1A regulates endocytosis, exocytosis, and focal adhesion turnover through spatial control of PI(4,5)P2 production.
The PIP5K1A gene is located on chromosome 1q21.3 and encodes a ubiquitously expressed enzyme enriched in the plasma membrane and perinuclear regions. PIP5K1A functions together with its isoforms PIP5K1B and PIP5K1C to maintain distinct pools of PI(4,5)P2 for different cellular processes. It interacts with small GTPases such as ARF6 and Rac1 to coordinate signaling at the membrane-cytoskeleton interface. Dysregulation of PIP5K1A disrupts cell polarity, vesicle trafficking, and growth factor signaling, contributing to diseases such as cancer and neurodegeneration.
In cell signaling, PIP5K1A modulates receptor-mediated activation of PI3K and PLC pathways, influencing calcium flux, membrane potential, and cytoskeletal remodeling. Its role in actin polymerization makes it essential for cell migration and adhesion dynamics. PIP5K1A activity is also linked to insulin signaling, where it regulates GLUT4 vesicle trafficking and glucose uptake in adipocytes and muscle cells.
PIP5K1A antibody is widely used in cell signaling, membrane biology, and lipid metabolism research. It is suitable for immunoblotting, immunofluorescence, and lipid kinase assays to analyze PIP5K1A expression and localization. This antibody aids in studying phosphoinositide regulation, cytoskeletal remodeling, and endocytic trafficking. In disease research, it supports investigations into cancer metastasis, metabolic regulation, and neuronal plasticity.
Structurally, PIP5K1A contains an N-terminal kinase domain with ATP- and substrate-binding sites, regulatory motifs for localization, and autophosphorylation sites that control activity.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
