| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PIP5K1B recombinant protein (Position: R394-L540) was used as the immunogen for the PIP5K1B antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PIP5K1B Antibody / Phosphatidylinositol-4-phosphate 5-kinase type 1 beta is a anti-PIP5K1B Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PIP5K1B
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PIP5K1B is encoded by the PIP5K1B gene on human chromosome 9q34.11. The protein is approximately 540 amino acids long and localizes mainly to the plasma membrane, with additional pools at the Golgi and endosomal compartments. As a member of the phosphatidylinositol-4-phosphate 5-kinase type I family, PIP5K1B works in concert with its isoforms PIP5K1A and PIP5K1C to sustain local lipid signaling domains that control cytoskeletal remodeling and vesicular trafficking.
The PIP5K1B antibody detects a 68 kilodalton band by western blot and shows strong plasma-membrane enrichment under immunofluorescence microscopy. PIP5K1B contributes to the organization of stress fibers and focal adhesions by controlling actin polymerization. It also modulates membrane ruffling, clathrin-mediated endocytosis, and vesicle docking. In neurons, PIP5K1B maintains synaptic vesicle recycling and presynaptic architecture by sustaining phosphatidylinositol-4,5-bisphosphate-dependent protein interactions.
Mutations or reduced expression of PIP5K1B have been associated with parkinsonism-dystonia syndromes, where impaired phosphoinositide metabolism leads to defective membrane trafficking and axonal degeneration. Dysregulation of PIP5K1B activity also contributes to cancer cell invasion through enhanced focal-adhesion turnover and migration. As a central regulator of lipid-signaling microdomains, PIP5K1B acts as a molecular hub linking mechanical and biochemical cues at the cell membrane.
Because PIP5K1B integrates phospholipid metabolism with cytoskeletal regulation, it provides insight into how membrane signaling orchestrates cellular architecture and motility.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
