| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PIPOX recombinant protein (Position: E173-L390) was used as the immunogen for the PIPOX antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PIPOX Antibody / Peroxisomal sarcosine oxidase is a anti-PIPOX Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Peroxisome).
Key elements and design rationale
- Target: PIPOX
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ELISA
Biological background
Functionally, PIPOX antibody identifies a 438-amino-acid peroxisomal matrix enzyme that catalyzes the conversion of sarcosine to glycine, formaldehyde, and hydrogen peroxide. PIPOX contains a covalently bound FAD cofactor that mediates electron transfer to molecular oxygen during oxidative demethylation. It also oxidizes L-pipecolate, a lysine degradation intermediate, linking it to amino acid metabolism and detoxification of secondary amines.
The PIPOX gene is located on chromosome 17p13.3 and encodes a protein expressed in liver, kidney, and muscle tissues, where peroxisomal metabolism is active. PIPOX contributes to maintaining amino acid balance and redox stability by coupling substrate oxidation with reactive oxygen species generation. In peroxisomes, PIPOX works alongside catalase to neutralize hydrogen peroxide, preventing oxidative stress accumulation.
Physiologically, PIPOX supports one-carbon metabolism by producing glycine, a precursor for glutathione synthesis and methylation reactions. Its regulation is influenced by dietary amino acid intake, metabolic state, and oxidative stress levels. Reduced PIPOX activity disrupts amino acid degradation and increases susceptibility to oxidative injury. Elevated activity has been observed in metabolic adaptations to fasting and in response to peroxisome proliferator-activated receptor (PPAR) signaling.
PIPOX antibody is widely used in peroxisomal biology, amino acid metabolism, and redox regulation research. It is suitable for western blotting, immunohistochemistry, and enzyme localization assays to study PIPOX expression and function. This antibody supports studies of peroxisomal oxidative pathways, redox signaling, and metabolic adaptation. In biomedical research, PIPOX serves as a marker of peroxisomal enzymatic activity and amino acid turnover.
Structurally, PIPOX contains FAD-binding and substrate-binding domains typical of the flavin oxidase family. It localizes to peroxisomes via a C-terminal peroxisomal targeting signal (PTS1).
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
