| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PITPNC1 recombinant protein (Position: E59-E332) was used as the immunogen for the PITPNC1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PITPNC1 Antibody / Phosphatidylinositol transfer protein cytoplasmic 1 is a anti-PITPNC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PITPNC1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Phosphatidylinositol transfer protein, cytoplasmic 1 binds phosphatidylinositol (PI) and phosphatidic acid (PA) and facilitates lipid exchange between Golgi and plasma membranes. It helps maintain phosphoinositide composition essential for vesicle budding and membrane identity. The PITPNC1 antibody supports localization studies revealing its enrichment at Golgi membranes and cytoplasmic vesicles. Through regulation of phosphoinositide pools, PITPNC1 coordinates signaling events that control secretion and membrane remodeling.
Recent research has shown that elevated PITPNC1 expression enhances tumor cell secretion and metastasis by increasing Golgi activity and secretion of pro-invasive factors such as MMP1 and HTRA1. The PITPNC1 antibody is a crucial reagent for investigating these mechanisms and assessing protein levels in metastatic cancer tissues. Its detection enables evaluation of how lipid signaling supports tumor cell migration and invasion through altered vesicle trafficking.
Phosphatidylinositol transfer protein, cytoplasmic 1 also functions in insulin secretion and lipid homeostasis, highlighting its importance beyond oncology. The PITPNC1 antibody supports studies into these metabolic processes by enabling detection in endocrine cells and liver tissues. Altered PITPNC1 expression may influence lipid droplet formation and phosphatidylinositol-dependent signaling in metabolic diseases.
The PITPNC1 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, providing characteristic Golgi-associated staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
