| Field | Specification |
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| Host | |
| Immunogen | A synthesized peptide derived from human PKC delta + PKC theta was used as the immunogen for the PKC delta/theta Antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
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Overview
PKC delta/theta Antibody / PRKCD/PRKCQ is a anti-PKC Rabbit antibody Recombinant Rabbit Monoclonal clone 31P77 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PKC
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31P77, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
PKC delta/theta antibody is widely applied in immunology, cancer biology, and signal transduction studies. PKC delta acts as a tumor suppressor or promoter depending on context, influencing apoptosis, DNA damage responses, and cell cycle regulation. PKC theta is essential for T cell activation, NF kappa B signaling, and cytokine production. By detecting both isoforms, researchers can evaluate overlapping and distinct functions in immunity, cancer, and tissue homeostasis.
Applications for PKC delta/theta antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect PKC isoforms in immune and cancer cell lysates, immunohistochemistry maps expression in tissues, and immunofluorescence highlights subcellular localization at membranes and cytoskeleton. These methods support detailed characterization of PKC signaling dynamics.
PKC delta contributes to apoptosis in response to DNA damage and oxidative stress, while PKC theta mediates survival signals in T cells. Dysregulated activity of either isoform contributes to autoimmunity, cancer progression, and cardiovascular disease. By applying PKC delta/theta antibody, scientists can examine how combined PKC signaling influences disease outcomes and therapeutic responses.
PKC isoforms are therapeutic targets, with inhibitors and modulators under development for cancer and immune disorders. Monitoring PKC delta and PKC theta expression provides biomarkers for pathway activity. PKC delta/theta antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
