| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3; KPYM_HUMAN |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Purified recombinant human PKM2 protein fragments expressed in E.coli. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyThe PKM2 (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1.
Validated Applications
| Western Blot (WB) | 1:500~1:1000 |
|---|---|
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Mouse |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Molecular Weight | ~ 56 kDa |
| Catalog No. | MB0189 |
| UniProt | P14618 |
| Alternative Names | PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/M2; Pyruvate kinase liver and blood cell; Pyruvate kinase liver and RBC; Pyruvate kinase liver and RBC type; Pyruvate kinase M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; Pyruvate kinase type L; R type/L type pyruvate kinase; Red cell/liver pyruvate kinase; RPK; TCB; THBP 1; THBP1; Thyroid hormone binding protein cytosolic; CTHBP; Cytosolic thyroid hormone binding protein; MGC3932; OIP 3; Oip3; Tumor M2-PK; p58; OIP-3; KPYM_HUMAN |
Immunogen / Antigen
Purified recombinant human PKM2 protein fragments expressed in E.coli.
Reactivity Notes
PKM2 monoclonal antibody detects endogenous levels of PKM2 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:500~1:1000 IF: 1:50~1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat, Monkey. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Purified recombinant human PKM2 protein fragments expressed in E.coli.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
