| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PKN3 recombinant protein (Position: E2-P889) was used as the immunogen for the PKN3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PKN3 Antibody / Protein kinase N3 is a anti-PKN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasmic, Nuclear.
Key elements and design rationale
- Target: PKN3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Structurally, PKN3 contains an N-terminal regulatory region with HR1 (homology region 1) motifs that bind Rho GTPases, followed by a C-terminal catalytic domain similar to protein kinase C (PKC). PKN3 phosphorylates substrates involved in actin filament stabilization and cell junction assembly, coordinating mechanical and adhesive cues. It is localized to the cytoplasm and plasma membrane, where it interacts with focal adhesion proteins and scaffolds to transmit signals influencing motility and growth.
The PKN3 antibody is widely used in cancer biology, cell signaling, and cytoskeletal research to investigate Rho-dependent kinase pathways and cell morphology control. Western blot analysis detects a 110 kilodalton band corresponding to PKN3, while immunofluorescence shows diffuse cytoplasmic and membrane-associated staining in epithelial and tumor cells. This antibody supports research into the molecular basis of migration, invasion, and metastatic progression.
PKN3 is overexpressed in several cancers, including breast, prostate, and pancreatic carcinomas, where it promotes invasive behavior through activation of the PI3K/AKT and RhoA pathways. Genetic or pharmacologic inhibition of PKN3 suppresses tumor growth and metastasis, making it a promising therapeutic target. Beyond oncology, PKN3 also contributes to endothelial cell barrier function and angiogenesis. The PKN3 antibody provides a valuable reagent for dissecting the cellular functions and therapeutic potential of this kinase.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
