| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human RARRES3/PLAAT4 recombinant protein (Position: M1-K124) was used as the immunogen for the PLAAT4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PLAAT4 Antibody / RARRES3 is a anti-PLAAT4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PLAAT4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, ELISA
Biological background
Structurally, PLAAT4 is an approximately 18 kilodalton enzyme localized to the endoplasmic reticulum and Golgi membranes. It contains a conserved catalytic dyad characteristic of the NlpC/P60 thiol protease superfamily, which mediates both phospholipase A and acyltransferase activities. Through its dual enzymatic function, PLAAT4 regulates cellular lipid composition, affecting membrane curvature, vesicle trafficking, and immune response modulation. Its expression is upregulated by retinoic acid and interferons, suggesting a role in antiviral defense and differentiation control.
The PLAAT4 antibody is widely used in cancer biology, immunology, and lipid signaling research to study retinoid-responsive pathways and lipid remodeling. Western blot analysis identifies a band at approximately 18 kilodaltons corresponding to PLAAT4, while immunofluorescence reveals perinuclear and vesicular localization consistent with endoplasmic reticulum distribution. This antibody enables investigation of how lipid hydrolysis and acylation influence inflammatory signaling and cellular differentiation.
Functionally, PLAAT4 acts as a tumor suppressor by antagonizing the Wnt/beta-catenin pathway, suppressing proliferation, and promoting differentiation in epithelial tissues. It also participates in the interferon-mediated antiviral response, where its enzymatic products help modulate viral replication and immune activation. In lipid metabolism, PLAAT4 contributes to the synthesis of signaling lipids that regulate inflammation and apoptosis. Dysregulation or loss of PLAAT4 expression has been observed in cancers such as breast and colorectal carcinoma, linking it to impaired retinoid signaling and altered lipid homeostasis. The PLAAT4 antibody provides a reliable reagent for exploring these cellular processes and identifying therapeutic targets in retinoid and immune pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
