| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PLBD2 recombinant protein (Position: Q108-Q525) was used as the immunogen for the PLBD2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PLBD2 Antibody / Phospholipase B domain-containing protein 2 is a anti-PLBD2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human. Reported localization: Cytoplasm (lysosome).
Key elements and design rationale
- Target: PLBD2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): ELISA, FACS, IF, IHC, ICC, WB
Biological background
Functionally, PLBD2 antibody recognizes a 516-amino-acid protein localized primarily in lysosomes and the endoplasmic reticulum. PLBD2 contains a conserved phospholipase B-like catalytic domain, which may mediate hydrolysis of membrane phospholipids and lysophospholipids. By modulating lipid composition, PLBD2 contributes to vesicle formation, endosomal trafficking, and lipid droplet metabolism. Its expression is induced by inflammatory cytokines and lipid stress, indicating a regulatory role in lipid homeostasis and immune signaling.
The PLBD2 gene is located on chromosome 12q13.13 and encodes a protein that shares structural features with classical phospholipases but displays distinct substrate specificity. While its catalytic mechanism remains partially defined, studies suggest that PLBD2 may participate in lipid recycling and detoxification under stress conditions. Proteomic analyses have identified PLBD2 as part of the lysosomal proteome, implicating it in membrane degradation and recycling of lipid components.
In immunology, PLBD2 has been linked to macrophage activation and inflammatory signaling. It may function as a lipid-modifying enzyme that influences phagosome maturation and pathogen clearance. Dysregulation of PLBD2 expression has been observed in metabolic disorders and cancers, suggesting that it plays a broader role in cellular metabolism and immune modulation. Elevated PLBD2 levels correlate with altered phospholipid profiles and increased autophagic activity in response to nutrient deprivation.
PLBD2 antibody is employed in lipidomics, immunology, and cell biology studies to investigate lysosomal function and lipid signaling pathways. It is useful for immunoblotting, immunofluorescence, and proteomic profiling of organelle-specific proteins. The enzyme's localization in the lysosomal compartment makes it an important marker for studies of membrane dynamics and lipid degradation.
Structurally, PLBD2 contains the conserved GXGXXG motif characteristic of lipid-hydrolyzing enzymes and a putative signal peptide for targeting to intracellular membranes. Its domain organization suggests roles in lipid recognition and hydrolysis. Post-translational regulation may involve glycosylation and proteolytic processing that modulate catalytic efficiency. Ongoing research continues to clarify its enzymatic specificity and physiological relevance in lipid remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
