| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PLCB2 recombinant protein (Position: D27-L1185) was used as the immunogen for the PLCB2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PLCB2 Antibody / Phospholipase C-beta-2 is a anti-PLCB2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PLCB2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 is widely expressed in hematopoietic and epithelial cells. It interacts with G alpha q and G beta gamma subunits of heterotrimeric G-proteins, linking receptor activation to intracellular calcium mobilization. The PLCB2 antibody enables detection of this enzyme in tissues where rapid signal transduction is required, such as immune cells and neurons. Its enzymatic activity is central to processes like secretion, chemotaxis, and proliferation.
PLCB2 plays a pivotal role in innate and adaptive immunity by coupling receptor engagement to downstream effector responses. The PLCB2 antibody supports investigations into Fc receptor signaling, platelet activation, and leukocyte migration. Dysregulation of phospholipase C beta-2 can lead to aberrant immune signaling, inflammation, and altered calcium dynamics that contribute to autoimmune or cardiovascular disorders.
In addition to its immune functions, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 contributes to sensory transduction in olfactory and gustatory pathways. The PLCB2 antibody is therefore valuable for neuroscience and sensory-biology research. Knockout models reveal its necessity in taste signaling and mucosal immune defense.
The PLCB2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, typically revealing cytoplasmic and membrane-associated staining.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
