| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PLCD1 recombinant protein (Position: Q14-R549) was used as the immunogen for the PLCD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PLCD1 Antibody / Phospholipase C delta 1 is a anti-PLCD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PLCD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, PLCD1 antibody identifies a 756-amino-acid enzyme containing an N-terminal PH domain for membrane binding, EF-hand motifs for calcium interaction, a catalytic X-Y domain for phosphodiesterase activity, and a C2 domain for phospholipid association. Upon receptor stimulation, PLCD1 catalyzes the cleavage of PIP2, generating IP3 to release intracellular calcium and DAG to activate protein kinase C (PKC).
The PLCD1 gene is located on chromosome 3p22.3 and is expressed in epithelial tissues, brain, and smooth muscle. It functions downstream of receptor tyrosine kinases and G protein-coupled receptors to mediate signal transduction. PLCD1 contributes to actin cytoskeleton remodeling and cell migration through localized phosphoinositide metabolism.
Pathologically, downregulation or mutation of PLCD1 is associated with cancer progression, skin disorders, and developmental defects. PLCD1 acts as a tumor suppressor in certain epithelial cancers by modulating calcium signaling and cell adhesion. Research using PLCD1 antibody supports studies in signal transduction, lipid metabolism, and cell communication.
PLCD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect phospholipase enzymes in signaling pathways.
Structurally, Phospholipase C delta 1 contains catalytic and regulatory domains that coordinate substrate recognition and calcium-dependent activity. This antibody facilitates investigation of PLCD1's role in intracellular signaling and calcium homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
