| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PLEKHB1 recombinant protein (Position: H82-S214) was used as the immunogen for the PLEKHB1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PLEKHB1 Antibody / Pleckstrin homology domain-containing family B member 1 is a anti-PLEKHB1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Monkey, Mouse, Rat.
Key elements and design rationale
- Target: PLEKHB1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, PLEKHB1 contains a conserved PH domain that binds phosphatidylinositol lipids, anchoring the protein to the inner leaflet of the plasma membrane. It also possesses regions that mediate protein-protein interactions with cytoskeletal and vesicular trafficking partners. PLEKHB1 belongs to the pleckstrin homology domain-containing protein family, which includes PLEKHB2, and participates in membrane dynamics and receptor recycling. Co-localization studies show PLEKHB1 distributed along synaptic membranes and photoreceptor outer segments.
Functionally, PLEKHB1 plays an important role in synaptic vesicle trafficking and receptor localization. In the retina, it interacts with proteins such as syntaxin and SNAP25 to regulate photoreceptor membrane stability and neurotransmitter release. In neurons, it supports axonal transport and signal transduction by organizing lipid domains within the plasma membrane. PLEKHB1 may also contribute to cellular polarity through coordination of phosphoinositide signaling at the membrane. During development, PLEKHB1 expression coincides with neural and retinal differentiation.
Dysregulation or mutation of PLEKHB1 has been linked to inherited retinal degenerations and neurological disorders. Its loss disrupts photoreceptor membrane architecture, leading to vision impairment. In cancer biology, PLEKHB1 expression patterns have been used as markers of epithelial polarity and tumor differentiation. Pathway associations include membrane trafficking, phosphatidylinositol signaling, and synaptic vesicle exocytosis. Co-localization with other PH domain proteins suggests a conserved role in regulating membrane-associated signaling complexes.
The PLEKHB1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
