| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PLS1 recombinant protein (Position: E14-L581) was used as the immunogen for the PLS1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PLS1 Antibody / Plastin-1 is a anti-PLS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: PLS1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
Structurally, Plastin-1 is a 630-amino-acid cytoskeletal protein of approximately 68 kilodaltons containing two tandem calponin homology (CH) domains responsible for actin binding and two EF-hand calcium-binding motifs that regulate its activity. The protein localizes predominantly to actin-rich structures such as microvilli and adherens junctions, where it bundles filamentous actin into parallel arrays that reinforce cell shape and polarity.
The PLS1 antibody is widely used in cell biology, auditory physiology, and epithelial research to study cytoskeletal architecture, microvillar dynamics, and actin filament regulation. Western blot analysis detects a 68 kilodalton band corresponding to Plastin-1, while immunofluorescence demonstrates strong staining along apical membranes in epithelial cells. This antibody provides a reliable tool for investigating actin organization and microvillar maintenance.
Functionally, Plastin-1 is critical for maintaining the structural stability of brush border and sensory epithelia by linking actin filaments into tightly packed bundles. Mutations in PLS1 cause autosomal recessive nonsyndromic hearing loss due to disruption of stereocilia structure and function in cochlear hair cells. In intestinal cells, PLS1 supports nutrient absorption and epithelial resilience under mechanical stress. The PLS1 antibody supports studies of cytoskeletal integrity, epithelial morphogenesis, and hereditary hearing loss.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
