| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PMF1 was used as the immunogen for the PMF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PMF1 Antibody / Polyamine modulated factor 1 is a anti-PMF1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P63 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PMF1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P63, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IP
Biological background
Polyamine modulated factor 1 was originally identified for its ability to influence the binding of transcription factors to DNA in the presence of polyamines. Research using PMF1 antibody has demonstrated that it plays an essential role in regulating gene expression by modulating Nrf2 activity and controlling antioxidant response elements. This pathway is central to cellular defense against oxidative damage and environmental stressors, and PMF1 activity is critical in fine-tuning this response.
Beyond its role in transcription, PMF1 has been associated with chromatin organization. Studies with PMF1 antibody have shown that it interacts with histone modifying enzymes, suggesting a role in epigenetic regulation. By influencing both transcription factors and chromatin state, Polyamine modulated factor 1 integrates multiple layers of gene expression control. These findings expand its importance beyond a cofactor to a regulator of genome activity.
PMF1 has also been implicated in cancer. Research using PMF1 antibody has revealed altered expression in several tumor types, including lung, colon, and liver cancers. Elevated levels of PMF1 may enhance antioxidant defenses in tumor cells, promoting survival under stress conditions. Conversely, reduced expression may impair cellular responses to oxidative injury. These dual roles highlight its potential as a biomarker and therapeutic target.
PMF1 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects endogenous protein levels, immunohistochemistry highlights nuclear localization in tissue samples, and immunofluorescence demonstrates dynamic changes after oxidative stress. These applications make PMF1 antibody indispensable for transcription and stress research.
By supplying validated PMF1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
