| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PNKP recombinant protein (Position: D43-E487) was used as the immunogen for the PNKP antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PNKP Antibody / Polynucleotide kinase phosphatase is a anti-PNKP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PNKP
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
PNKP is encoded by the PNKP gene on human chromosome 19q13.33. The protein is approximately 521 amino acids long and contains an N-terminal forkhead-associated (FHA) domain that mediates interactions with XRCC1 and XRCC4, scaffolding proteins in the single-strand and double-strand break repair complexes. The C-terminal catalytic region includes both phosphatase and kinase domains that process DNA termini with abnormal 3'-phosphate or 5'-hydroxyl ends.
The PNKP antibody detects a 57 kilodalton band by western blot and shows nuclear foci following DNA damage. PNKP plays a dual role in the repair of oxidative and radiation-induced DNA breaks by preparing strand ends for gap filling and ligation. Mutations in PNKP cause neurological disorders such as microcephaly with early-onset seizures and developmental delay, resulting from defective DNA repair in postmitotic neurons.
PNKP activity is tightly regulated by phosphorylation and interaction with repair complexes. In cancer, overexpression of PNKP enhances DNA repair capacity, contributing to therapy resistance. Pharmacologic inhibition of PNKP sensitizes tumor cells to radiation and chemotherapeutic agents, underscoring its potential as a therapeutic target.
Because PNKP maintains genomic stability and neuronal survival, it serves as a key model for studying the interface between DNA repair and neurodegeneration.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
