| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human POC1A recombinant protein (Position: A6-D371) was used as the immunogen for the POC1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POC1A Antibody / POC1 centriolar protein A is a anti-POC1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: POC1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
POC1A is encoded by the POC1A gene located on human chromosome 3p21.2. The protein is approximately 45 kilodaltons and contains an N-terminal WD40 domain that mediates protein-protein interactions and a C-terminal coiled-coil region required for centriolar localization. POC1A localizes to the proximal ends of centrioles and basal bodies, where it contributes to centriole duplication fidelity and stability.
The POC1A antibody detects a 45 kilodalton band in western blot assays and shows centrosomal staining in interphase and mitotic cells. POC1A is required for the maintenance of mature centrioles and proper spindle orientation during cell division. It works in concert with other centriolar components, including CEP135 and SAS6, ensuring correct microtubule organization and ciliogenesis.
Mutations in POC1A cause primordial dwarfism and growth retardation syndromes due to defective centriole biogenesis and impaired mitotic progression. Cells lacking POC1A exhibit abnormal centrosome numbers, fragmented spindles, and cell cycle arrest. POC1A expression is also implicated in cilia assembly, linking centrosome function to signaling pathways such as Hedgehog and Wnt.
Because of its importance in centrosome biology, POC1A serves as a useful marker for studying mitotic structures, developmental abnormalities, and ciliopathies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
