| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human POLD4 recombinant protein (Position: M1-L107) was used as the immunogen for the POLD4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLD4 Antibody / DNA polymerase delta subunit 4 is a anti-POLD4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Nuclear.
Key elements and design rationale
- Target: POLD4
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Structurally, POLD4 is a ~12 kDa protein with no catalytic activity but essential stabilizing functions. It interacts with the catalytic subunit POLD1 and accessory subunits POLD2 and POLD3, forming the tetrameric polymerase delta complex. By supporting proper assembly, POLD4 ensures high-fidelity DNA replication and repair.
Functionally, POLD4 contributes to DNA replication, mismatch repair, and recombination. It coordinates lagging strand synthesis, working with FEN1 and DNA ligase I to process Okazaki fragments. Researchers use POLD4 antibody to study DNA replication, checkpoint control, and repair pathways.
Clinically, defects in DNA polymerase delta subunits including POLD4 have been linked to cancer, immunodeficiency, and genomic instability syndromes. Reduced POLD4 expression impairs DNA replication fidelity, contributing to mutagenesis. Because DNA replication is a key therapeutic target, POLD4 is being investigated in oncology.
Experimentally, POLD4 antibody is applied in western blotting to detect the ~12 kDa protein, in immunoprecipitation to isolate the polymerase delta complex, and in immunofluorescence to study nuclear localization. Chromatin immunoprecipitation with POLD4 antibody supports research on replication fork progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
