| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human POLR1A recombinant protein (Position: E607-R1720) was used as the immunogen for the POLR1A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLR1A Antibody / DNA-directed RNA polymerase I subunit RPA1 is a anti-POLR1A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: POLR1A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
Structurally, POLR1A contains a DNA-binding cleft, catalytic residues within the active center, and multiple contact surfaces for interacting with transcription factors and other RNA polymerase I subunits. It forms the backbone of the enzyme and interacts with RPA2 and RPA3 to establish the core complex. POLR1A also associates with transcription initiation factors such as SL1 and UBF, which guide polymerase recruitment to rDNA promoters and regulate transcriptional output in response to growth signals.
The POLR1A antibody is widely used in transcription, cell growth, and cancer biology research to study ribosomal biogenesis and nucleolar function. Western blot analysis detects a 190 kilodalton band corresponding to POLR1A, while immunofluorescence reveals strong nucleolar staining consistent with its role in rRNA synthesis. This antibody supports studies of transcriptional regulation, nucleolar stress responses, and metabolic control of protein synthesis.
POLR1A activity is tightly linked to cellular growth and proliferation. Under conditions of nutrient abundance, mTOR signaling promotes RNA polymerase I activation and rRNA transcription; conversely, DNA damage or stress inhibits POLR1A function to conserve resources. Mutations in POLR1A cause ribosomopathies such as acrofacial dysostosis, characterized by craniofacial malformations due to impaired ribosome production. Overactivation of RNA polymerase I, on the other hand, contributes to oncogenic transformation by driving excessive ribosome biogenesis and protein synthesis. The POLR1A antibody allows precise monitoring of polymerase expression and localization under these physiological and pathological conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
