| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) was used as the immunogen for the POLR1E antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLR1E Antibody / DNA-directed RNA polymerase I subunit E / PAF53 is a anti-POLR1E Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.
Key elements and design rationale
- Target: POLR1E
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, ELISA
Biological background
POLR1E localizes to the nucleolus and associates with the transcription initiation factor SL1 and upstream binding factor (UBF) to form a preinitiation complex at rDNA promoters. Its C-terminal domain mediates binding to RNA polymerase I subunit RPA1 and other core enzyme components, facilitating enzyme assembly and promoter clearance. Loss of POLR1E disrupts nucleolar architecture and reduces rRNA synthesis, leading to impaired ribosome production and cell-cycle arrest. This makes it a critical determinant of nucleolar integrity and growth control.
The POLR1E antibody is widely used in molecular biology, cancer research, and nucleolar studies to assess RNA polymerase I activity and rRNA transcription regulation. Western blot analysis identifies a 53 kilodalton band corresponding to POLR1E, while immunofluorescence reveals punctate nucleolar staining. Because RNA polymerase I transcription drives ribosome production, alterations in POLR1E expression are linked to tumor growth and metabolic adaptation. Increased expression has been observed in rapidly dividing cells, whereas downregulation occurs under cellular stress or growth inhibition.
In addition to its role in transcription, POLR1E contributes to the nucleolar stress response by coordinating signaling between rRNA synthesis and p53 activation. Defects in polymerase I assembly or activity trigger nucleolar stress pathways that stabilize p53, resulting in growth arrest. The POLR1E antibody enables exploration of these mechanisms and supports investigations into ribosomopathies, tumorigenesis, and cellular aging.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
