| Field | Specification |
|---|---|
| Alternative Names | DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; EC=2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; Short name=RPC155; RNA polymerase III subunit C160; POLR3A |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyPOLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome.
Validated Applications
| Western Blot (WB) | 1:500~1:1000 |
|---|---|
| Immunohistochemistry (IHC) | 1:50~1:200 |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 156 kDa |
| Catalog No. | BS3292 |
| UniProt | O14802 |
| Alternative Names | DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1; EC=2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; Short name=RPC155; RNA polymerase III subunit C160; POLR3A |
Immunogen / Antigen
Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A.
Reactivity Notes
POLR3A (P68) polyclonal antibody detects endogenous levels of POLR3A protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:500~1:1000 IHC: 1:50~1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Synthetic peptide, corresponding to amino acids 41-90 of Human POLR3A.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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