| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Amino acids K231-L360 from the human protein were used as the immunogen for the POLR3D antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
POLR3D Antibody / DNA-directed RNA polymerase III subunit RPC4 is a anti-POLR3D Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA, Immunofluorescence (IF) with listed reactivity in Human, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: POLR3D
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P, FACS, ELISA, IF
Biological background
DNA-directed RNA polymerase III subunit RPC4 contributes to the conserved catalytic core of the enzyme, providing structural stability and facilitating transcriptional activity. Its interactions with other polymerase subunits form part of the framework that enables RNA chain initiation and elongation. Beyond its basal role, POLR3D helps modulate transcription in response to signaling pathways, linking environmental and metabolic cues to RNA production.
Mutations in DNA-directed RNA polymerase III subunit RPC4 have been associated with rare neurodevelopmental syndromes, underscoring its importance in human biology. Furthermore, RNA polymerase III and its subunits, including POLR3D, are closely tied to innate immune function. Viral infections often exploit RNA polymerase III activity, while host defenses detect RNA polymerase III products as part of antiviral recognition systems. This highlights the dual role of POLR3D in normal physiology and immune response.
Cancer research has also implicated RNA polymerase III dysregulation, where increased transcription of tRNAs and 5S rRNA drives uncontrolled protein synthesis and cellular proliferation. As part of this complex, DNA-directed RNA polymerase III subunit RPC4 may contribute to tumor development by supporting excessive biosynthetic activity. Ongoing studies are examining whether POLR3D and its associated pathways can serve as biomarkers or therapeutic targets.
The POLR3D antibody is applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to assess expression, localization, and changes in disease or experimental models. These tools are vital for researchers investigating transcriptional regulation, immune surveillance, and oncogenic pathways. For advanced molecular studies requiring consistent and accurate detection, the POLR3D antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
