Polycystin 1 Antibody / PKD1

Overview

Polycystin 1 Antibody / PKD1 is an antibody targeting PKD1, raised in Goat for protein detection and localization studies where these specifications are required.

Key elements and design rationale

• Target: PKD1.
• Antibody identity: Polyclonal (goat origin); Goat Ig.
• Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
• Format: Antigen affinity purified.
• Species reactivity: Human.
• Listed applications: IHC-P, ELISA (peptide) (refer to on-page specifications for application-specific guidance).

Biological background

Polycystin 1, also known as PKD1, is a protein encoded by the PKD1 gene and is crucial in the development of Polycystic Kidney Disease (PKD). PKD1 mutations are responsible for the majority of cases of autosomal dominant PKD, a genetic disorder that causes the formation of fluid-filled cysts in the kidneys. Polycystin 1 is a transmembrane protein that plays a vital role in cell-cell and cell-matrix interactions. It is primarily expressed in the kidneys, liver, pancreas, and other tissues, where it helps regulate cell proliferation, differentiation, and apoptosis. Dysfunction of Polycystin 1 disrupts these essential cellular processes, leading to the formation of cysts in the kidneys. Research has shown that Polycystin 1 interacts with other proteins, such as Polycystin 2, to form a protein complex that regulates calcium signaling and cell growth. Mutations in PKD1 disrupt this complex, causing aberrant calcium signaling and uncontrolled cell proliferation, which ultimately results in cyst formation and kidney damage. Studies have also suggested that Polycystin 1 may play a role in the mechanosensory function of kidney epithelial cells, allowing them to respond to changes in fluid flow and pressure within the nephron. Dysregulation of this mechanosensory function due to PKD1 mutations may contribute to the development and progression of PKD.

Research relevance and current trends

• Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
• Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
• Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.

Common research applications

• Immunohistochemistry: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• ELISA: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.

Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.

Notes for experimental interpretation

• Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
• Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
• Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
• Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.