| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PPAR gamma was used as the immunogen for the PPAR gamma antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PPAR gamma Antibody / PPARG is a anti-PPAR Rabbit antibody Recombinant Rabbit Monoclonal clone 31P97 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: PPAR
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31P97, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, FACS
Biological background
PPAR gamma exists in at least two isoforms, PPAR gamma 1 and PPAR gamma 2, which differ in tissue distribution and regulatory control. PPAR gamma 1 is broadly expressed, while PPAR gamma 2 is more adipocyte specific. Both isoforms are activated by endogenous ligands such as prostaglandin derivatives and polyunsaturated fatty acids, as well as synthetic ligands like thiazolidinediones. These synthetic agonists have been widely used as antidiabetic medications, making PPAR gamma antibody an important reagent in metabolic disease research.
The receptor is also implicated in cancer biology. PPAR gamma can regulate cell cycle arrest, apoptosis, and differentiation. Studies using PPAR gamma antibody have shown altered expression in breast, colon, and prostate cancers. Inflammation research has further demonstrated that PPAR gamma modulates immune responses by suppressing pro inflammatory cytokine production in macrophages and T cells.
Detection of PPAR gamma with PPAR gamma antibody is routinely carried out in western blotting, immunohistochemistry, and chromatin immunoprecipitation. Western blotting reveals isoform specific expression, while immunohistochemistry highlights nuclear localization. Chromatin immunoprecipitation assays combined with next generation sequencing have uncovered PPAR gamma binding sites across the genome, providing insights into transcriptional networks controlling energy homeostasis.
PPAR gamma remains a therapeutic target for type 2 diabetes, obesity, and cardiovascular disease. Researchers rely on PPAR gamma antibody to study receptor expression, ligand responsiveness, and downstream signaling changes under physiological and pathological conditions. Availability of validated antibodies such as those from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
