| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PPIH recombinant protein (Position: M1-M177) was used as the immunogen for the PPIH antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PPIH Antibody / Peptidyl-prolyl cis-trans isomerase H is a anti-PPIH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PPIH
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, ELISA
Biological background
Functionally, PPIH antibody identifies a 177-amino-acid protein localized primarily in the nucleus, where it associates with the spliceosome machinery. PPIH is a component of the U4/U6.U5 tri-snRNP complex, interacting with PRPF4, SNU13, and other splicing factors. Through its isomerase activity, PPIH facilitates conformational rearrangements necessary for spliceosome assembly and catalysis. Beyond its role in RNA splicing, PPIH also contributes to protein folding and trafficking, reflecting its general chaperone-like activity within the cyclophilin family.
The PPIH gene is located on chromosome 1p34.2 and encodes a protein containing a single cyclophilin-type peptidyl-prolyl isomerase (PPIase) domain. PPIH is structurally similar to other cyclophilins such as PPIA and PPIL1 but exhibits specialized interactions within the spliceosome. Its enzymatic activity accelerates folding of substrates containing cis-proline bonds, influencing the conformation of RNA-binding proteins and enzymes involved in gene expression.
Loss or inhibition of PPIH function affects pre-mRNA splicing efficiency and fidelity, leading to transcriptome-wide alterations. Dysregulation of PPIH expression has been observed in cancer and neurodegenerative diseases, suggesting roles in RNA metabolism and stress response. In cell signaling, PPIH interacts with transcription and export factors, linking splicing to mRNA maturation and nuclear export.
PPIH antibody is used to study RNA processing, protein folding, and chaperone function. It is applicable for immunoblotting, immunoprecipitation, and fluorescence microscopy to examine PPIH localization and protein-protein interactions. In structural biology, PPIH serves as a model enzyme for studying cyclophilin catalytic mechanisms. Its sensitivity to cyclosporine A highlights its functional conservation across cyclophilins and its potential involvement in immunomodulatory pathways.
Structurally, PPIH features a conserved beta-barrel fold typical of cyclophilins, with a hydrophobic active site pocket that accommodates the proline-containing peptide bond. It is post-translationally regulated by phosphorylation and complex assembly within spliceosomal subunits.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
