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| Immunogen | A synthesized peptide derived from human PPM1G was used as the immunogen for the PPM1G antibody. |
| Isotype | |
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Overview
PPM1G Antibody / Protein phosphatase 1G is a anti-PPM1G Rabbit antibody Recombinant Rabbit Monoclonal clone 29P73 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PPM1G
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29P73, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
Protein phosphatase 1G has been implicated in regulation of the G2/M transition of the cell cycle. Research using PPM1G antibody has shown that depletion of this phosphatase delays mitotic entry and leads to accumulation of phosphorylated cyclin dependent kinase substrates. By reversing phosphorylation of key regulators, PPM1G ensures proper timing of mitotic onset. Its involvement in cell cycle checkpoints makes it critical for maintaining genomic stability and preventing uncontrolled proliferation.
PPM1G also plays a role in DNA damage responses. Studies using PPM1G antibody have demonstrated that it dephosphorylates histone H2AX and other chromatin associated substrates, facilitating repair and chromatin restoration following DNA double strand breaks. This highlights its function as a chromatin associated phosphatase linking signaling pathways to epigenetic control. Dysregulation of PPM1G expression or activity may impair DNA repair, contributing to mutagenesis and cancer susceptibility.
In addition to DNA repair and cell cycle regulation, PPM1G is involved in pre mRNA splicing. By dephosphorylating components of the spliceosome, it regulates splicing factor dynamics and mRNA maturation. Research with PPM1G antibody has revealed that it controls alternative splicing patterns and transcriptional responses, linking phosphatase activity to gene expression. This broadens its functional impact beyond canonical phosphatase roles and highlights its integration into multiple layers of gene regulation.
PPM1G antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting demonstrates expression across proliferative tissues, while immunohistochemistry localizes the phosphatase in nuclei of dividing cells. Immunoprecipitation with PPM1G antibody isolates its complexes, enabling characterization of substrates and interaction partners. Together, these approaches make PPM1G antibody an important tool for studying signaling, genome maintenance, and transcriptional regulation.
By supplying validated PPM1G antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
