| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human iASPP/PPP1R13L recombinant protein (Position: D94-Q753) was used as the immunogen for the PPP1R13L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PPP1R13L Antibody / iASPP / Protein phosphatase 1 regulatory subunit 13 like is a anti-PPP1R13L Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PPP1R13L
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Functionally, PPP1R13L antibody identifies a 918-amino-acid nuclear and cytoplasmic protein that binds to the DNA-binding domain of p53, preventing the activation of pro-apoptotic genes such as BAX and PUMA. PPP1R13L also interacts with the RelA/p65 subunit of NF-kappaB, inhibiting its transcriptional activity and modulating inflammatory signaling. Through its association with PP1, it influences the phosphorylation state of nuclear proteins, linking phosphatase activity to transcriptional repression.
The PPP1R13L gene is located on chromosome 19q13.32 and is expressed in multiple tissues, including heart, liver, and brain, with elevated expression in dividing cells and tumors. Expression is induced by growth factors and oncogenic signals, reflecting its role in balancing survival and stress responses. PPP1R13L is part of the ASPP (apoptosis-stimulating proteins of p53) family, which fine-tunes p53 function depending on cellular context.
Pathologically, overexpression of PPP1R13L contributes to oncogenesis by suppressing p53-dependent apoptosis and enhancing cell survival under stress. High levels are found in breast, liver, and hematologic malignancies. Conversely, reduced expression sensitizes cells to apoptosis. Genetic variants have also been linked to cardiomyopathy and developmental abnormalities. Research using PPP1R13L antibody supports studies in apoptosis regulation, tumor biology, and transcriptional control.
PPP1R13L antibody can be validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect transcriptional regulators.
Structurally, Protein phosphatase 1 regulatory subunit 13 like contains ankyrin repeats and an SH3 domain that mediate interactions with p53 and NF-kappaB, as well as PP1-binding motifs for phosphatase association. This modular structure allows PPP1R13L to serve as a multifunctional scaffold integrating transcriptional repression and survival signaling. This antibody supports investigation of PPP1R13L's role in cell fate determination, stress response, and tumor progression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
