| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PPP1R8 recombinant protein (Position: L27-N312) was used as the immunogen for the PPP1R8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PPP1R8 Antibody / Protein phosphatase 1 regulatory subunit 8 is a anti-PPP1R8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: PPP1R8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IF, FACS, ELISA
Biological background
PPP1R8 is encoded by the PPP1R8 gene on human chromosome 1p35.1. The protein contains a central PP1-binding motif (RVXF) that mediates association with PP1, a forkhead-associated (FHA) domain involved in phosphoprotein recognition, and a nuclear localization signal. PPP1R8 functions as both an inhibitor and targeting subunit of PP1, directing its activity toward specific nuclear substrates such as splicing factors and transcriptional regulators. It also participates in the regulation of cell cycle progression and DNA replication by modulating the phosphorylation state of histones and replication proteins.
Studies using the PPP1R8 antibody have shown that the protein localizes predominantly to the nucleus, with strong accumulation in speckle-like structures associated with RNA splicing. Western blot analysis typically detects bands near 35-40 kDa corresponding to the primary isoform, although multiple splice variants exist. PPP1R8 interacts with CDC5L, SNW1, and other components of the spliceosome complex, underscoring its integral role in pre-mRNA processing. In addition to RNA metabolism, PPP1R8 regulates transcription by interacting with Polycomb repressive complex proteins and influencing histone methylation states.
Functionally, PPP1R8 acts as a checkpoint in cellular stress responses and may modulate signaling cascades involving Akt and MAP kinases. Dysregulation has been implicated in cancer and neurodegenerative disorders through its impact on protein phosphorylation balance and gene expression.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
