| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PPP2R5D was used as the immunogen for the PPP2R5D antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PPP2R5D Antibody / Protein phosphatase 2 regulatory subunit B delta is a anti-PPP2R5D Rabbit antibody Recombinant Rabbit Monoclonal clone 30P07 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PPP2R5D
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P07, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
PPP2R5D antibody is widely applied in neuroscience, developmental biology, and cancer research. Mutations in PPP2R5D cause neurodevelopmental disorders characterized by intellectual disability, hypotonia, macrocephaly, and autism spectrum features. By detecting PPP2R5D, researchers can study how alterations in PP2A signaling contribute to brain development and cognitive function. In cancer biology, PP2A acts as a tumor suppressor, and dysregulation of its regulatory subunits can promote oncogenesis.
PPP2R5D directs PP2A activity toward key regulators such as Akt, MAPK, and MYC, influencing pathways that control proliferation and apoptosis. The antibody supports studies into how these signaling cascades are altered by mutations or changes in expression. Detection of PPP2R5D provides insight into phosphatase signaling networks that are otherwise difficult to measure directly.
Western blot assays detect PPP2R5D protein in neuronal and cancer cell lysates. Immunohistochemistry maps tissue expression in brain, liver, and tumors. Immunofluorescence highlights nuclear or cytoplasmic localization depending on signaling context. These tools provide comprehensive approaches for studying PP2A regulation.
PPP2R5D is implicated in rare genetic disorders grouped as PPP2R5D related neurodevelopmental syndromes. These conditions result from de novo mutations and highlight the critical role of PP2A signaling in neurodevelopment. By using PPP2R5D antibody, scientists can explore genotype phenotype correlations and mechanisms underlying disease pathology.
In oncology, dysregulated PP2A contributes to leukemia, breast cancer, and colorectal cancer. Altered expression of regulatory subunits like PPP2R5D changes phosphatase balance, leading to sustained oncogenic signaling. Therapeutic strategies are exploring reactivation of PP2A complexes as a tumor suppressor approach. PPP2R5D antibody therefore supports both mechanistic studies and translational drug development.
PPP2R5D antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
