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| Immunogen | A synthesized peptide derived from human PPP2R5E was used as the immunogen for the PPP2R5E antibody. |
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Overview
PPP2R5E Antibody / Serine/threonine-protein phosphatase 2A regulatory subunit B epsilon is a anti-PPP2R5E Rabbit antibody Recombinant Rabbit Monoclonal clone 30P55 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PPP2R5E
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P55, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF
Biological background
Serine/threonine-protein phosphatase 2A regulatory subunit B'epsilon directs PP2A activity toward specific signaling pathways, including those governing cell cycle progression, apoptosis, and cytoskeletal organization. Research with PPP2R5E antibody has demonstrated its role in directing PP2A complexes to centrosomes, kinetochores, and other subcellular structures, where it contributes to mitotic spindle formation and chromosome segregation. By targeting defined substrates, PPP2R5E ensures that PP2A exerts context dependent regulatory effects rather than indiscriminate dephosphorylation.
Aberrant regulation of PP2A, including dysfunction of regulatory subunits such as PPP2R5E, has been linked to cancer, neurodegeneration, and cardiovascular disease. Loss of PP2A activity promotes oncogenesis by enhancing phosphorylation driven signaling pathways such as PI3K AKT and MAPK. Studies employing PPP2R5E antibody have shown that altered expression of this subunit contributes to tumorigenesis and drug resistance. In the nervous system, deregulated PP2A activity is implicated in tau hyperphosphorylation and neurodegenerative conditions including Alzheimer disease. PPP2R5E antibody provides a means to explore these dysfunctions and to assess whether restoration of PP2A activity can provide therapeutic benefit.
In addition to disease connections, PPP2R5E regulates developmental and metabolic processes. It influences insulin signaling, cardiac function, and immune responses, highlighting its broad biological significance. Research using PPP2R5E antibody has revealed that PP2A complexes containing this regulatory subunit can fine tune signaling thresholds, allowing cells to balance growth and survival. This underscores the precision with which PP2A holoenzymes must be assembled to maintain cellular homeostasis.
PPP2R5E antibody is widely used in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting detects expression patterns in dividing cells and tumors, while immunohistochemistry reveals tissue specific expression across brain, muscle, and immune organs. Immunoprecipitation with PPP2R5E antibody allows isolation of PP2A complexes containing this regulatory subunit, enabling analysis of substrate interactions and signaling outcomes. These approaches ensure that PPP2R5E antibody is versatile across molecular and cellular applications.
By supplying validated PPP2R5E antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
