| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PRDM11 recombinant protein (Position: E37-L483) was used as the immunogen for the PRDM11 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PRDM11 Antibody / PR domain-containing protein 11 is a anti-PRDM11 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: PRDM11
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, FACS, ELISA
Biological background
PRDM11 functions as a transcriptional repressor that modulates gene expression by recruiting histone-modifying enzymes such as histone methyltransferases and HDACs. Through these interactions, PRDM11 establishes repressive chromatin environments to silence developmental regulators. It plays a role in maintaining proper differentiation states of B cells and neurons by controlling genes involved in proliferation and signaling. Co-localization studies show PRDM11 concentrated in nuclear foci where transcriptional repression occurs.
Structurally, PRDM11 contains an N-terminal PR/SET domain that is homologous to histone lysine methyltransferases, although its intrinsic enzymatic activity remains unclear. The C-terminal region includes multiple zinc finger motifs that recognize specific DNA sequences and allow recruitment of co-repressors. It belongs to the PRDM family of transcriptional regulators, which includes PRDM1 (BLIMP-1), PRDM2, and PRDM16, all of which play pivotal roles in development and oncogenesis.
Functionally, PRDM11 contributes to chromatin-mediated gene silencing and developmental programming. It represses target genes involved in cell proliferation and signaling pathways, promoting stable cell identity. In neural tissues, PRDM11 is implicated in neuronal subtype specification, while in hematopoietic cells it influences differentiation and germinal center responses. It may also coordinate cross-talk between chromatin remodeling complexes and transcriptional repressors such as REST and HDAC1.
Dysregulation of PRDM11 is associated with lymphoma and other cancers. Loss of expression or chromosomal rearrangements involving PRDM11 are reported in diffuse large B-cell lymphoma, suggesting a tumor suppressor function. Aberrant PRDM11 activity can also disrupt neural differentiation or germ cell maturation. Pathway associations include chromatin organization, histone modification, and transcriptional repression networks. During embryogenesis, PRDM11 expression correlates with organogenesis and tissue patterning.
Immunohistochemical staining using PRDM11 antibody reveals nuclear localization in lymphocytes and neuronal cells. The PRDM11 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
