| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PRDM6 recombinant protein (Position: R165-R452) was used as the immunogen for the PRDM6 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PRDM6 Antibody / PR domain zinc finger protein 6 is a anti-PRDM6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PRDM6
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
PR domain zinc finger protein 6 functions as a transcriptional repressor by recruiting histone methyltransferases and corepressor complexes, leading to trimethylation of histone H3 on lysine 9 (H3K9me3) and gene silencing. It is preferentially expressed in smooth muscle�derived lineages where it regulates genes controlling contractile protein synthesis and cell fate. The PRDM6 antibody provides a reliable means to monitor expression changes during phenotypic switching of VSMCs between contractile and proliferative states, a process central to vascular injury response and atherosclerosis.
Loss-of-function mutations in PRDM6 have been associated with patent ductus arteriosus (PDA) and other congenital heart defects. These variants impair transcriptional repression of target genes involved in vascular maturation. The PRDM6 antibody facilitates investigation into such pathogenic mechanisms by allowing detection of wild-type and mutant protein forms. In developmental studies, PR domain zinc finger protein 6 has been shown to coordinate the balance between smooth muscle proliferation and differentiation during embryogenesis, acting downstream of BMP and TGF-beta signaling pathways.
Beyond cardiovascular biology, PR domain zinc finger protein 6 has been implicated in oncogenesis through its role in epigenetic gene silencing. Aberrant PRDM6 expression or methylation patterns have been observed in multiple cancer types, suggesting that dysregulation of this transcriptional repressor contributes to uncontrolled cell growth. The PRDM6 antibody enables detailed exploration of its epigenetic regulatory activity in cancer and stem cell systems, providing insight into how histone modification and chromatin structure impact disease progression.
The PRDM6 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing predominant nuclear localization consistent with its transcriptional role.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
