PRKCD Antibody

Overview

PRKCD Antibody is a research-use antibody directed against PRKCD. It is supplied for use in common immunoassay contexts such as WB, IHC-P, IF FACS, Direct ELISA (RUO).

Key elements and design rationale

• Target: PRKCD.
• Description (provided): Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene.
• Antibody type: Rabbit, Polyclonal (rabbit origin), Rabbit IgG.
• Format: Antigen affinity purified; Affinity purified.
• Reported/predicted localization: Cytoplasmic, nuclear, plasma membrane.
• Species reactivity: tested: Human, Mouse, Rat.
• Immunogen (if provided): A human recombinant protein (amino acids F4-D676) was used as the immunogen for the PRKCD antibody..

The information above helps you match the antibody format to your assay context, interpret species-dependent differences, and anticipate how epitope context (isoforms, PTMs, or conformational state) may influence signal.

Biological background

Protein kinase C delta type, also called PKCD, is an enzyme that in humans is encoded by the PRKCD gene. The PRKCD gene encodes a member of the protein kinase C family, members of which are critical for regulation of cell survival, proliferation, and apoptosis. This gene is mapped to 3p21.1. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. PRKCD is also regulated by phosphorylation on various tyrosine residues including Y311 (by SRC). It has been showed that PRKCD phosphorylates NLRC4 and that this phosphorylation is critical for inflammasome assembly. It is also a part of a noncanonical WNT signaling cascade.

For curated annotations (gene/protein naming, domains, isoforms, and pathway links) for PRKCD, consult primary databases such as UniProt, NCBI Gene, and Ensembl.

Research relevance and current trends

• Context-dependent expression studies: researchers often examine PRKCD abundance and localization across perturbations (genetic, pharmacologic, or environmental) to connect phenotype to molecular changes.
• Reagent reproducibility: there is growing emphasis on antibody specificity checks using orthogonal approaches (e.g., genetic perturbation or independent antibodies) and transparent reporting of clone/lot information.
• Multi-modal datasets: antibody-based readouts are increasingly combined with transcriptomics and imaging to relate protein-level measurements to cell-state transitions.

Common research applications

• Western blotting (immunoblot) for relative detection of target protein abundance and apparent molecular weight.
• Immunohistochemistry for spatial mapping of target expression across tissues and cell types.
• Immunofluorescence for subcellular localization and cell-type specific expression patterns.
• Direct ELISA: commonly used to detect or compare PRKCD across experimental conditions (conceptual guidance only).

When comparing conditions, interpret changes in signal in the context of sample composition, expected localization, and any known isoform complexity for the target.

Notes for experimental interpretation

• Isoforms and PTMs: alternative splicing or post-translational modifications can change epitope accessibility and apparent molecular weight; interpret bands/signals accordingly.
• Cross-reactivity and matrix effects: background binding can vary by sample type, species, and blocking/detection chemistries; include appropriate negative controls.
• Control concepts: where feasible, use genetic perturbation (KO/KD/overexpression), orthogonal assays, or independent antibodies to support specificity claims.

Antibody considerations: Polyclonal reagents may recognize multiple epitopes and can increase sensitivity but may show broader binding profiles, while monoclonal clones provide a single-epitope readout that can improve consistency across experiments. If a conjugate is listed, the antibody supports more direct detection workflows; otherwise, it is typically used with a compatible secondary antibody.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.