| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant chimeric protein (sequences within amino acids 180-380 and 612-765) was used as the immunogen for the Prominin-1 antibody. This Prominin-1 antibody binds both glycosylated and non-glycosylated extracellular domains of Prominin-1. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
Prominin-1 Antibody / PROM1 / CD133 is an antibody targeting Prominin-1. Host: Mouse. Monoclonal. Clone: PROM/1510. Format: Purified. Applications: IHC-P.
Key elements and design rationale
- Target: Prominin-1.
- Immunogen: Recombinant chimeric protein (sequences within amino acids 180-380 and 612-765) was used as the immunogen for the Promi….
- Species reactivity: Human.
- Localization: Cytoplasm (ER).
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein.
Common research applications
- IHC: tissue distribution assessment; interpret with matched controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.