| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PRP4K recombinant protein (Position: P94-I1007) was used as the immunogen for the PRP4K antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PRP4K Antibody / Pre-mRNA-processing factor 4B kinase is a anti-PRP4K Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear.
Key elements and design rationale
- Target: PRP4K
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
PRP4K antibody targets a nuclear kinase that associates with spliceosomal components and regulates phosphorylation of splicing factors such as SF3B1 and PRP6, influencing spliceosome assembly and mRNA maturation. PRP4K activity is essential for accurate exon recognition and alternative splicing decisions, affecting transcriptome diversity. The protein also modulates mitotic checkpoint signaling through interaction with BUB1 and MAD1 complexes, ensuring proper chromosome segregation. Dysregulation of PRPF4B expression has been associated with aberrant splicing events, genomic instability, and tumorigenesis.
The PRPF4B gene is located on chromosome 6p21.31 and encodes a protein of approximately 1007 amino acids containing an N-terminal kinase domain and a C-terminal proline-rich regulatory region. PRP4K phosphorylates SR-rich splicing proteins, coordinating RNA processing with transcriptional elongation and stress responses. Its activity is regulated by phosphorylation, autoinhibition, and interactions with 14-3-3 proteins. Elevated expression of PRP4K has been observed in cancers such as breast, ovarian, and glioblastoma, where it influences epithelial-mesenchymal transition (EMT) and drug resistance phenotypes.
Researchers employ PRP4K antibody for western blotting, immunofluorescence, and immunoprecipitation to monitor nuclear localization, post-translational modifications, and spliceosomal associations. The kinase also participates in response to genotoxic stress and growth factor signaling via ERK and JNK cascades. Functional depletion studies reveal that loss of PRP4K impairs cell cycle progression and enhances sensitivity to DNA-damaging agents. Because PRP4K integrates splicing control with cell survival signaling, it has emerged as a potential therapeutic target for cancer intervention.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
