| Field | Specification |
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| Mfr No | |
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| Host | |
| Immunogen | A synthesized peptide derived from human PRPF31 was used as the immunogen for the PRPF31 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
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| Target | |
| UniProt # |
Overview
PRPF31 Antibody / Pre-mRNA processing factor 31 is a anti-PRPF31 Rabbit antibody Recombinant Rabbit Monoclonal clone 29P97 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: PRPF31
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29P97, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, ICC, IF, FACS
Biological background
Pre-mRNA processing factor 31 is indispensable for the assembly of the U4/U6.U5 tri-snRNP, which integrates into the spliceosome during pre-mRNA splicing. Research using PRPF31 antibody has demonstrated that loss of this protein disrupts tri-snRNP stability, preventing proper spliceosome activation and leading to defective intron removal. This highlights the importance of PRPF31 in maintaining gene expression fidelity and explains why its mutations cause tissue-specific disease despite its general role in splicing.
Mutations in PRPF31 are a well-established cause of autosomal dominant retinitis pigmentosa, a degenerative eye disease characterized by progressive photoreceptor cell death and vision loss. Studies with PRPF31 antibody have shown that reduced protein expression, often due to haploinsufficiency, leads to insufficient splicing activity in retinal cells. This impairs photoreceptor survival and contributes to disease progression. The link between PRPF31 and retinal degeneration underscores the importance of splicing factors in tissue-specific pathologies.
Beyond retinitis pigmentosa, alterations in splicing machinery are increasingly recognized in cancer and neurodegeneration. PRPF31 antibody has been used to examine expression in tumors, where splicing factor mutations or dysregulation contribute to aberrant gene expression. In neurological disease, spliceosome defects are emerging as contributors to motor neuron degeneration and other pathologies. Studying Pre-mRNA processing factor 31 provides insight into how splicing fidelity influences cell fate and disease susceptibility.
PRPF31 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms expression levels across tissues, immunohistochemistry highlights nuclear localization, and immunofluorescence shows colocalization with spliceosome markers. Functional studies using PRPF31 antibody include knockdown and rescue experiments to establish causal relationships between reduced PRPF31 and splicing defects. These diverse applications make it an indispensable tool for RNA biology research.
By providing validated PRPF31 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
