| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PRPF4 was used as the immunogen for the PRPF4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PRPF4 Antibody / Pre mRNA processing factor 4 is a anti-PRPF4 Rabbit antibody Recombinant Rabbit Monoclonal clone 30P52 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.
Key elements and design rationale
- Target: PRPF4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30P52, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
PRPF4 antibody is highly relevant for studies of gene expression regulation and RNA biology. By detecting PRPF4, researchers can investigate how spliceosome components assemble and how splicing is regulated under different physiological conditions. Disruption of PRPF4 function impairs RNA processing and leads to widespread changes in gene expression, highlighting its importance in cell biology.
The antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and other molecular biology applications. In western blot assays, PRPF4 antibody recognizes nuclear protein bands, confirming expression across tissue and cell samples. Immunohistochemistry provides tissue localization, often highlighting nuclear distribution in actively transcribing cells. Immunofluorescence further resolves subnuclear localization and colocalization with other spliceosomal proteins.
Mutations in PRPF4 are linked to retinitis pigmentosa, a degenerative retinal disorder characterized by progressive vision loss. This connection underscores the physiological importance of splicing factors beyond general RNA metabolism. PRPF4 antibody therefore provides a valuable reagent for studies of inherited retinal disease as well as broader investigations of splicing related disorders.
Splicing dysregulation is also increasingly recognized in cancer and neurodegeneration. Aberrant splicing contributes to oncogenic transformation, resistance to therapy, and progression of neurodegenerative diseases. Detecting PRPF4 expression with specific antibodies enables researchers to connect splicing factor function with disease mechanisms and to evaluate therapeutic interventions targeting the spliceosome.
PRPF4 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
