| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human PSIP1 / LEDGF was used as the immunogen for the PSIP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSIP1 Antibody / PC4 and SFRS1-interacting protein / LEDGF is a anti-PSIP1 Rabbit antibody Recombinant Rabbit Monoclonal clone 31P66 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.
Key elements and design rationale
- Target: PSIP1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31P66, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF
Biological background
Lens epithelium derived growth factor is best known for its role in HIV infection, where it acts as a tethering factor for integrase, directing viral DNA integration into active chromatin regions. Research using PSIP1 antibody has confirmed that depletion of LEDGF reduces viral replication efficiency, underscoring its importance in retroviral life cycles. Beyond virology, PSIP1 regulates stress response genes, including those activated by heat shock and oxidative stress, contributing to cellular resilience. By detecting this protein with PSIP1 antibody, researchers gain insight into how cells survive hostile environments.
PSIP1 also influences development and cancer. Overexpression of LEDGF has been observed in leukemia and solid tumors, where it enhances survival pathways and confers resistance to apoptosis. Chromosomal translocations involving PSIP1 result in fusion proteins that act as oncogenic drivers, particularly in mixed lineage leukemia. Studies employing PSIP1 antibody have demonstrated altered transcriptional programs and stress resistance in tumor cells, linking LEDGF to oncogenesis and therapeutic resistance. Because of these functions, PSIP1 remains a focus in drug discovery and biomarker research.
PSIP1 antibody is applied in western blotting, chromatin immunoprecipitation, and immunohistochemistry. Western blotting demonstrates isoform expression and stress inducible regulation. Chromatin immunoprecipitation with PSIP1 antibody identifies genomic targets of LEDGF, revealing pathways controlled by this coactivator. Immunohistochemistry highlights tissue distribution, while functional assays demonstrate its ability to modulate transcriptional activity under stress. These diverse applications underscore the importance of PSIP1 antibody in molecular and cellular research.
By supplying validated PSIP1 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
