| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Gankyrin/PSMD10 recombinant protein (Position: M1-G226) was used as the immunogen for the PSMD10 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSMD10 Antibody / Gankyrin is a anti-PSMD10 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PSMD10
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Gankyrin contains multiple ankyrin repeats that mediate protein–protein interactions with proteasome subunits and cell cycle regulators. It associates with CDK4, MDM2, and the 26S proteasome base complex to coordinate protein turnover. By enhancing MDM2-mediated ubiquitination of p53, 26S proteasome non-ATPase regulatory subunit 10 reduces tumor suppressor activity and contributes to cellular transformation. The PSMD10 antibody enables direct detection of Gankyrin expression in tumor tissues and cultured cells, supporting studies of its oncogenic potential and correlation with clinical outcomes.
Overexpression of Gankyrin has been observed in numerous cancers, including hepatocellular carcinoma, colorectal carcinoma, and breast cancer. It accelerates cell cycle progression, promotes resistance to apoptosis, and enhances metastatic potential. The PSMD10 antibody allows researchers to quantify expression levels and subcellular localization of this protein, helping elucidate its functional role in oncogenic signaling pathways. Downregulation or inhibition of 26S proteasome non-ATPase regulatory subunit 10 has been shown to restore p53 stability and suppress tumor growth, making it an appealing therapeutic target.
Beyond its oncogenic properties, Gankyrin contributes to proteasome assembly and quality control. It stabilizes interactions between ATPase and non-ATPase subunits of the 19S regulatory particle, facilitating efficient protein degradation. The PSMD10 antibody is suitable for western blotting, immunoprecipitation, and immunohistochemistry, allowing characterization of proteasomal function in normal and malignant cells. Its application extends to studies of proteostasis, cell cycle control, and ubiquitin-mediated degradation pathways.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
