| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PSTPIP1 recombinant protein (Position: M1-D394) was used as the immunogen for the PSTPIP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PSTPIP1 Antibody / PEST phosphatase-interacting protein 1 is a anti-PSTPIP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: PSTPIP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
PSTPIP1 is encoded by the PSTPIP1 gene located on human chromosome 15q24.3. The protein belongs to the F-BAR domain-containing family and features an N-terminal F-BAR domain that mediates membrane curvature and actin binding, along with a C-terminal SH3 domain that interacts with proline-rich regions in partner proteins such as PEST-type tyrosine phosphatases and pyrin. Through these interactions, PSTPIP1 regulates membrane deformation, phagocytosis, and signaling cascades in macrophages and neutrophils.
The PSTPIP1 antibody typically detects a 48-52 kilodalton protein by western blot and produces strong cytoplasmic and membrane-associated staining in immune cells. Functionally, PSTPIP1 links the actin cytoskeleton to signaling complexes, modulating processes such as cell spreading, migration, and inflammasome assembly. Mutations in PSTPIP1 are associated with PAPA (pyogenic arthritis, pyoderma gangrenosum, and acne) syndrome and other autoinflammatory disorders, where altered protein-protein interactions lead to hyperactivation of the pyrin inflammasome and excessive interleukin-1 beta production.
PSTPIP1 interacts with proteins including WASP, c-Abl kinase, and SHIP1, integrating actin dynamics with immune receptor signaling. In macrophages, it contributes to the formation of podosomes and phagocytic structures. Beyond immune regulation, PSTPIP1 participates in bone remodeling and osteoclast differentiation through cytoskeletal control.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
