| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human PTCD1 recombinant protein (Position: D203-L601) was used as the immunogen for the PTCD1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
PTCD1 Antibody / Pentatricopeptide repeat domain-containing protein 1 is a anti-PTCD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Mitochondria.
Key elements and design rationale
- Target: PTCD1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
PTCD1 belongs to a large family of PPR proteins that are characterized by their modular RNA-binding repeats. In mammalian cells, PTCD1 localizes to the mitochondrial matrix and associates with mitochondrial ribonucleoprotein complexes. Functional studies indicate that PTCD1 modulates expression of key mitochondrial-encoded subunits of the respiratory chain, including components of complexes I and IV. Knockdown or loss of PTCD1 disrupts mitochondrial translation, leading to impaired ATP synthesis and increased reactive oxygen species production. As such, PTCD1 plays a central role in maintaining mitochondrial integrity and energy homeostasis.
The PTCD1 antibody is widely used in studies of mitochondrial biology, RNA processing, and metabolic regulation. In western blot assays, PTCD1 is detected as a ~75 kilodalton band, while immunofluorescence reveals punctate staining consistent with mitochondrial localization. Expression profiling has shown that PTCD1 is abundant in metabolically active tissues such as heart, skeletal muscle, and brain, where mitochondrial activity is high. The antibody is an essential reagent for examining mitochondrial gene expression pathways and for identifying alterations in mitochondrial function associated with metabolic disorders and neurodegeneration.
Beyond its core function in RNA metabolism, PTCD1 has been linked to mitochondrial-nuclear communication and apoptosis regulation. It may influence the transcription of nuclear-encoded mitochondrial genes by modulating signaling pathways that respond to mitochondrial dysfunction. Genetic variants of PTCD1 have been associated with susceptibility to metabolic diseases, including type 2 diabetes and mitochondrial encephalomyopathy. The PTCD1 antibody thus enables researchers to explore these regulatory networks and to define the molecular mechanisms that couple RNA metabolism with mitochondrial biogenesis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
