| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | PTEN; MMAC1; TEP1; Phosphatidylinositol 3; 4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PTEN around the phosphorylation site of Ser370. AA range:355-385 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage |
Product Overview
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate ener
Polyclonal Rabbit AntibodyValidated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot | Refer to datasheet |
| Immunohistochemistry | Refer to datasheet |
| Immunofluorescence | Refer to datasheet |
| ELISA | Refer to datasheet |
Immunogen & Specificity
Immunogen: The antiserum was produced against synthesized peptide derived from human PTEN around the phosphorylation site of Ser370. AA range:355-385
Subcellular Localization: Cytoplasm . Nucleus . Nucleus, PML body . Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620). XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982). .; [Isoform alpha]: Secreted . May be secreted via a classical signal peptide and reenter into cells with the help of a poly-Arg motif.
UniProt/Swiss-Prot: P60484
Product Specifications
| Host Species | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Storage | -20°C/1 year |
Reactivity Notes
Confirmed reactivity in Human, Mouse, Rat samples; validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA.
Safety & Handling
- For Research Use Only (RUO). Not intended for diagnostic procedures.
- Shipping: cold-chain shipment (typically with ice packs).
- Upon receipt: store at recommended temperature as soon as possible.
- Sales terms and conditions: Please review prior to ordering.
Related Products
Explore additional antibodies and related detection reagents for PTEN (phospho Ser370) in the BioHippo catalog.
This antibody has been validated for Western Blot, Immunohistochemistry, Immunofluorescence. Refer to the product datasheet for recommended dilutions and protocol guidance for each application.
Refer to the datasheet for recommended dilution ranges per application. Optimal dilution may vary by sample type, fixation method, and detection system — a titration experiment is recommended prior to full experimental use.
This antibody has been validated or predicted to recognize Human, Mouse, Rat samples, based on immunogen sequence homology. For unlisted species, contact our team for guidance.
This antibody was raised against a specific peptide or recombinant protein fragment corresponding to PTEN (phospho Ser370). Exact immunogen details are provided in the product datasheet.
Conjugated formats may be available upon request. Contact the BioHippo team for custom conjugation options including fluorophore, biotin, or enzyme labeling.
BioHippo can support custom antibody formatting and service options for selected products. Contact us to discuss your project requirements.
Potential customization options include:
- Custom conjugation: fluorophore, biotin, enzyme, or nanoparticle labeling available on request for qualified antibodies.
- Bulk or scale-up quantities: volume pricing and custom lead time estimates available for larger orders.
- Alternative formulations: azide-free, carrier protein-free, or lyophilized formats available upon request for selected products.
- OEM and private-label supply: custom packaging and branding available for commercial applications.
Submit your project specifications via the product contact form or reach us at support@ebiohippo.com for a tailored quotation.
